Two postprocessing techniques for the elimination of background autofluorescence for fluorescence lifetime imaging microscopy.
J Biomed Opt
; 13(1): 014008, 2008.
Article
de En
| MEDLINE
| ID: mdl-18315366
ABSTRACT
The analysis of fluorescence lifetime imaging microscopy (FLIM) data under complex biological conditions can be challenging. Particularly, the presence of short-lived autofluorescent aggregates can confound lifetime measurements in fluorescence energy transfer (FRET) experiments, where it can become confused with the signal from exogenous fluorophores. Here we report two techniques that can be used to discriminate the contribution of autofluorescence from exogenous fluorphores in FLIM. We apply the techniques to transgenic mice that natively express yellow fluorescence protein (YFP) in a subset of cortical neurons and to histological slices of aged human brain tissue, where we study the misfolding of intracellular tau protein in the form of neurofibrillary tangles.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Algorithmes
/
Interprétation d'images assistée par ordinateur
/
Amélioration d'image
/
Technique de soustraction
/
Artéfacts
/
Transfert d'énergie par résonance de fluorescence
/
Microscopie de fluorescence
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Limites:
Animals
Langue:
En
Journal:
J Biomed Opt
Sujet du journal:
ENGENHARIA BIOMEDICA
/
OFTALMOLOGIA
Année:
2008
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique