[Mechanism of recombinant adenovirus-mediated mutations of hypoxia inducible factor 1alpha in modulation of cell apoptosis].
Nan Fang Yi Ke Da Xue Xue Bao
; 28(3): 309-12, 2008 Mar.
Article
de Zh
| MEDLINE
| ID: mdl-18359679
ABSTRACT
OBJECTIVE:
To investigate the mechanism by which recombinant adenovirus (Ad)-mediated mutations of hypoxia inducible factor 1alpha (Ad-HIF-1alpha-Ala564-Ala803) regulates cell apoptosis.METHODS:
LoVo cells were infected with recombinant Ad-HIF-1alpha-Ala564-Ala803 and control virus Ad-lacZ under normoxia condition. Real-time PCR was used to detect HIF-1alpha and p21WAF1/CIP1 mRNA expressions at different time points. Western blotting was employed to verify HIF-1alpha and p21WAF1/CIP1 protein expression. Hoechst 33342 flourescein staining was performed to observe the ratio of apoptotic LoVo cells.RESULTS:
The expression levels of HIF-1alpha mRNA and protein increased after infection with Ad-HIF-1alpha- Ala564-Ala803, accompanied by an increase in p21WAF1/CIP1 mRNA and protein expressions. The apoptotic ratio was significantly higher in LoVo cells infected with recombinant Ad-HIF-1alpha-Ala564-Ala803 (16.2%) than in the control cells (5.5%, P=0.00).CONCLUSION:
HIF-1alpha may induce cell cycle arrest by up-regulating p21WAF1/CIP1 expressions to promote LoVo cell apoptosis.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Adenoviridae
/
Apoptose
/
Sous-unité alpha du facteur-1 induit par l'hypoxie
/
Mutation
Limites:
Humans
Langue:
Zh
Journal:
Nan Fang Yi Ke Da Xue Xue Bao
Année:
2008
Type de document:
Article
Pays d'affiliation:
Chine
Pays de publication:
CHINA
/
CN
/
REPUBLIC OF CHINA