Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum.
Mol Cell Neurosci
; 39(2): 229-37, 2008 Oct.
Article
de En
| MEDLINE
| ID: mdl-18652899
ABSTRACT
Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated synapses. Such broad action of transgene NPY may prevent recruitment of excitatory synapses in epileptic activity and could play a key role in limiting the spread and generalization of seizures.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Neuropeptide Y
/
Potentialisation à long terme
/
Acide glutamique
/
Transgènes
/
Hippocampe
Limites:
Animals
Langue:
En
Journal:
Mol Cell Neurosci
Sujet du journal:
BIOLOGIA MOLECULAR
/
NEUROLOGIA
Année:
2008
Type de document:
Article
Pays d'affiliation:
Suède