A novel 3D in vitro culture model to study stromal-epithelial interactions in the mammary gland.
Tissue Eng Part C Methods
; 14(3): 261-71, 2008 Sep.
Article
de En
| MEDLINE
| ID: mdl-18694322
ABSTRACT
Stromal-epithelial interactions mediate mammary gland development and the formation and progression of breast cancer. To study these interactions in vitro, the development of defined three-dimensional (3D) models is essential. In the present study, we have successfully developed novel 3D in vitro models that allow the formation of mammary gland structures closely resembling those found in vivo. Cocultures of a human mammary epithelial cell line MCF10A and human mammary fibroblasts obtained from reduction mammoplasties embedded in either a type I collagen or a mixed Matrigel-collagen matrix were carried out for up to 6 weeks. Histological and ultrastructural analysis confirmed the formation of ductal and alveolar structures. The importance of the stromal cells was apparent in both matrices; in the collagen gels the presence of reduction mammoplasty fibroblasts accelerated the initial formation of epithelial structures, and in the mixed Matrigel-collagen gels the presence of those fibroblasts was necessary for the formation of ductal structures. These models provide an excellent system to study tissue organization, epithelial morphogenesis, and breast carcinogenesis.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéoglycanes
/
Tumeurs du sein
/
Collagène
/
Laminine
/
Techniques de culture cellulaire
/
Ingénierie tissulaire
/
Glandes mammaires humaines
/
Fibroblastes
Limites:
Humans
Langue:
En
Journal:
Tissue Eng Part C Methods
Sujet du journal:
BIOTECNOLOGIA
/
HISTOLOGIA
Année:
2008
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique