Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans.

Taha, Taher M; Kanao, Tadayoshi; Takeuchi, Fumiaki; Sugio, Tsuyoshi

Taha, Taher M; Kanao, Tadayoshi; Takeuchi, Fumiaki; Sugio, Tsuyoshi.

Affiliation

Taha TM; Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. tahery1@yahoo.com

Appl Environ Microbiol ; 74(21): 6808-10, 2008 Nov.

Article de En | MEDLINE | ID: mdl-18791023

ABSTRACT

ABSTRACT

The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.

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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Oxidoreductases / Acidithiobacillus / Fer Langue: En Journal: Appl Environ Microbiol Année: 2008 Type de document: Article Pays d'affiliation: Japon

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