Localized surface plasmon coupled fluorescence fiber-optic biosensor for alpha-fetoprotein detection in human serum.
Biosens Bioelectron
; 24(6): 1610-4, 2009 Feb 15.
Article
de En
| MEDLINE
| ID: mdl-18823773
ABSTRACT
In this study, we demonstrated that the fiber-optic biosensor based on localized surface plasmon coupled fluorescence (LSPCF) is capable of detecting alpha-fetoprotein (AFP) in human serum. The sensitivity of LSPCF fiber-optic biosensor is not only enhanced but also the specific selectivity is improved since the fluorophores are excited by the localized surface plasmon with high efficiency. Experimentally, this fiber-optic biosensor is able to detect AFP concentration in phosphate buffered saline (PBS) solution from 0.1ng/mL to 100ng/mL whereas the linear relationship between the AFP concentrations and the fluorescence signals is shown. Furthermore, a linear response between the fluorescence signals and the concentrations of AFP in human serum from 2.33ng/mL to 143.74ng/mL is also obtained. As a result, the detection limit of the LSPCF fiber-optic biosensor on AFP detection is comparable with the conventional enzyme-linked immunosorbent assay (ELISA). Additionally, the LSPCF fiber-optic biosensor benefits on inexpensive, disposable and simpler optical geometry that can become a high efficient immunoassay comparable with the conventional ELISA and radioimmunoassay (RIA) clinically.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Spectrométrie de fluorescence
/
Analyse chimique du sang
/
Dosage immunologique
/
Techniques de biocapteur
/
Alphafoetoprotéines
/
Résonance plasmonique de surface
/
Technologie des fibres optiques
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Limites:
Humans
Langue:
En
Journal:
Biosens Bioelectron
Sujet du journal:
BIOTECNOLOGIA
Année:
2009
Type de document:
Article
Pays d'affiliation:
Taïwan