Your browser doesn't support javascript.
loading
DCDHF fluorophores for single-molecule imaging in cells.
Chemphyschem ; 10(1): 55-65, 2009 Jan 12.
Article de En | MEDLINE | ID: mdl-19025732
ABSTRACT
There is a persistent need for small-molecule fluorescent labels optimized for single-molecule imaging in the cellular environment. Application of these labels comes with a set of strict requirements strong absorption, efficient and stable emission, water solubility and membrane permeability, low background emission, and red-shifted absorption to avoid cell autofluorescence. We have designed and characterized several fluorophores, termed "DCDHF" fluorophores, for use in live-cell imaging based on the push-pull

design:

an amine donor group and a 2-dicyanomethylene-3-cyano-2,5-dihydrofuran (DCDHF) acceptor group, separated by a pi-rich conjugated network. In general, the DCDHF fluorophores are comparatively photostable, sensitive to local environment, and their chemistries and photophysics are tunable to optimize absorption wavelength, membrane affinity, and solubility. Especially valuable are fluorophores with sophisticated photophysics for applications requiring additional facets of control, such as photoactivation. For example, we have reengineered a red-emitting DCDHF fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. This molecule and its relatives provide a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control (here turning-on) of single-molecule emission.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Colorants fluorescents / Furanes / Nitriles Limites: Animals Langue: En Journal: Chemphyschem Sujet du journal: BIOFISICA / QUIMICA Année: 2009 Type de document: Article Pays d'affiliation: États-Unis d'Amérique

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Colorants fluorescents / Furanes / Nitriles Limites: Animals Langue: En Journal: Chemphyschem Sujet du journal: BIOFISICA / QUIMICA Année: 2009 Type de document: Article Pays d'affiliation: États-Unis d'Amérique
...