[Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 22(4): 287-9, 2008 Aug.
Article
de Zh
| MEDLINE
| ID: mdl-19105346
ABSTRACT
OBJECTIVE:
To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.METHODS:
TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.RESULTS:
TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.CONCLUSION:
HBX protein could be induced into mouse liver by TAT induced peptide.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Expression des gènes
/
Transactivateurs
/
Membrane cellulaire
/
Produits du gène tat du virus de l'immunodéficience humaine
/
Protéines virales régulatrices ou accessoires
Limites:
Animals
/
Female
/
Humans
/
Male
Langue:
Zh
Journal:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Sujet du journal:
VIROLOGIA
Année:
2008
Type de document:
Article
Pays d'affiliation:
Chine