Spore-forming organisms in platelet concentrates: a challenge in transfusion bacterial safety.
Transfus Med
; 18(6): 371-6, 2008 Dec.
Article
de En
| MEDLINE
| ID: mdl-19140821
ABSTRACT
Bacterial detection and pathogen reduction are widely used methods of minimizing the risk of transfusion-transmitted bacterial infection. But, bacterial spores are highly resistant to chemical and physical agents. In this study, we assessed the bacterial proliferation of spore-forming organisms seeded into platelet concentrates (PCs) to demonstrate that spores can enter the vegetative state in PCs during storage. In the in vitro study, PCs were inoculated with 1-10 spores mL(-1)of Bacillus cereus (n = 1), Bacillus subtilis (n = 2) and Clostridium sporogenes (n = 2). Sampling was performed during 6-day aerobic storage at 22 degrees C. The presence of bacteria was assessed by plating culture, automated culture and real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Spores of the C. sporogenes do not enter the vegetative phase under PC storage conditions, whereas B. subtilis and B. cereus showed growth in the PC and could be detected using RT-PCR and automated culture. Depending on the species and inoculums, bacterial spores may enter the vegetative phase during PC storage and can be detected by bacterial detection methods.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Bacillus cereus
/
Bacillus subtilis
/
Sang
/
Plaquettes
/
Clostridium
Limites:
Humans
Langue:
En
Journal:
Transfus Med
Sujet du journal:
HEMATOLOGIA
Année:
2008
Type de document:
Article
Pays d'affiliation:
Allemagne