The effect of rhG-CSF on the conformation of LFA-1 on CD4+ T cells in hemopoietic stem cell transplantation.
Immunopharmacol Immunotoxicol
; 31(2): 267-73, 2009 Jun.
Article
de En
| MEDLINE
| ID: mdl-19235605
ABSTRACT
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) modulates donor T cell function in hemopoietic stem cell transplantation. The effects of rhG-CSF on the activation of CD4(+) T cells have been poorly investigated. We investigated whether rhG-CSF mobilization influenced the activation and proliferation capacity of CD4(+) T cells. Cell treatment with phorbol 12-myristate 13-acetate (PMA) plus ionomycin or the CD3 mAb OKT3 plus intercellular cell adhesion molecule-1 (ICAM-1) at 37 degrees C for 6 h induced a dramatic increase in CD25, CD69 and MEM148 epitope exposure. rhG-CSF mobilization decreased CD25, CD69 and MEM148 epitope expression on activated CD4(+) T cells compared with cells before mobilization. The transcription factor Jun activation domain-binding protein 1 (JAB1) plays a role in the activation of CD4(+) T cells, and the rhG-CSF mobilization changed the level of nuclear JAB1 protein. rhG-CSF mobilization also decreased the adhesion of CD4(+) T cells to ICAM-1, but had no effect on the levels of donor CD4+CD25+ regulatory T cells. Overall, these data suggest the rhG-CSF mobilization can influence CD4(+) T cell activation through LFA-1/ICAM-1 costimulatory signaling in HSC transplantation.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lymphocytes T CD4/
/
Facteur de stimulation des colonies de granulocytes
/
Antigène-1 associé à la fonction du lymphocyte
/
Transplantation de cellules souches hématopoïétiques
Limites:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Langue:
En
Journal:
Immunopharmacol Immunotoxicol
Sujet du journal:
ALERGIA E IMUNOLOGIA
/
FARMACOLOGIA
/
TOXICOLOGIA
Année:
2009
Type de document:
Article
Pays d'affiliation:
Chine