Route to smooth silica-based surfaces decorated with novel self-assembled monolayers (SAMs) containing glycidyl-terminated very long hydrocarbon chains.
Langmuir
; 25(10): 5526-35, 2009 May 19.
Article
de En
| MEDLINE
| ID: mdl-19378931
ABSTRACT
Novel glycidyl-terminated organosilicon coupling agents possessing a trialkoxysilyl head group and a very long hydrocarbon chain (C22) were synthesized. Their ability to afford densely packed self-assembled monolayers (SAMs) grafted on silica-based surfaces was investigated. Transmission FT-IR spectra showed that the most regular films were obtained by using trichloracetic acid as the catalyst (10 M%). Atomic force microscopy (AFM) and optical ellipsometry were consistent with well ordered monolayers exhibiting a marked decrease of the surface roughness. Epifluorescence microscopy revealed that these SAMs possessed a better surface reactivity than monolayers obtained with the commercially available (3-glycidoxypropyl) trimethoxysilane (GPTS) upon grafting of a fluorescent probe (dansylcadaverin). Moreover, direct attachment of fluorescent antibodies (RAG-TRITC) through covalent binding led to higher mean fluorescence intensities, showing that these new SAMs possess high potential for the immobilization of biological molecules.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Silanes
/
Silice
/
Réactifs réticulants
/
Membrane artificielle
Langue:
En
Journal:
Langmuir
Sujet du journal:
QUIMICA
Année:
2009
Type de document:
Article
Pays d'affiliation:
France