Mst4 and Ezrin induce brush borders downstream of the Lkb1/Strad/Mo25 polarization complex.
Dev Cell
; 16(4): 551-62, 2009 Apr.
Article
de En
| MEDLINE
| ID: mdl-19386264
ABSTRACT
The human Lkb1 kinase, encoded by the ortholog of the invertebrate Par4 polarity gene, is mutated in Peutz-Jeghers cancer syndrome. Lkb1 activity requires complex formation with the pseudokinase Strad and the adaptor protein Mo25. The complex can induce complete polarization in a single isolated intestinal epithelial cell. We describe an interaction between Mo25alpha and a human serine/threonine kinase termed Mst4. A homologous interaction occurs in the yeast Schizosaccharomyces pombe in the control of polar tip growth. Human Mst4 translocates from the Golgi to the subapical membrane compartment upon activation of Lkb1. Inhibition of Mst4 activity inhibits Lkb1-induced brush border formation, whereas other aspects of polarity such as the formation of lateral junctions remain unaffected. As an essential event in brush border formation, Mst4 phosphorylates the regulatory T567 residue of Ezrin. These data define a brush border induction pathway downstream of the Lkb1/Strad/Mo25 polarization complex, yet separate from other polarity events.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de liaison au calcium
/
Polarité de la cellule
/
Protein-Serine-Threonine Kinases
/
Protéines du cytosquelette
/
Protéines adaptatrices du transport vésiculaire
/
Microvillosités
Limites:
Humans
Langue:
En
Journal:
Dev Cell
Sujet du journal:
EMBRIOLOGIA
Année:
2009
Type de document:
Article
Pays d'affiliation:
Pays-Bas