Determination of tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations by HPLC.
Int J Cosmet Sci
; 31(6): 445-50, 2009 Dec.
Article
de En
| MEDLINE
| ID: mdl-19467029
ABSTRACT
A rapid HPLC method was developed for the assay of tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations. The validated method was applied for quantitative determination of these vitamins in simulated emulsion formulation. Samples were analysed directly on a RP-18 reverse phase column with UV detection at 222 nm. A mixture of methanol and isopropanol (25 75 v/v) was used as mobile phase. The retention time of tocopheryl acetate and ascorbyl tetraisopalmitate were 3.0 min and 5.9 min, respectively. Recovery was between 95% and 104%. In addition, the excipients did not interfere in the analysis. The method is simple, reproducible, selective and is suitable for routine analyses of commercial products.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Acide ascorbique
/
Cosmétiques
/
Tocophérols
/
Antioxydants
Langue:
En
Journal:
Int J Cosmet Sci
Année:
2009
Type de document:
Article
Pays d'affiliation:
Brésil