Nucleotide and amino acid homology between the human thyrotropin receptor and the HIV-1 Nef protein: identification and functional analysis.
Biochem Biophys Res Commun
; 181(1): 498-505, 1991 Nov 27.
Article
de En
| MEDLINE
| ID: mdl-1958218
ABSTRACT
A comparison of the nucleotide sequence of the human thyrotropin receptor (hTSH-R) with that of HIV-1 revealed 61% homology between a 161 base pair region encoding a unique portion of the hTSH-R and an immunogenic HIV-1 regulatory protein, nef. Amino acid analysis of this region shows 27% homology, including a segment in which 7/10 consecutive amino acids are identical. Sera from rabbits successfully immunized with a 16 amino acid portion of the hTSH-R (352-367, p1) was assessed for reactivity against a partially homologous nef peptide (nef-1) by ELISA, with a finding five-fold higher post-immunization values compared to pre-immune sera. The specificity of this response was verified with Western blot, using recombinant nef protein. An ELISA using nef-1 gave 64% higher values with sera from Graves' disease patients than with normal controls. This homology and immunologic cross-reactivity suggests an avenue through which a shared immune response against an HIV-1 related retrovirus could play a role in the pathogenesis of Graves' disease.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Récepteur TSH
/
Produits du gène nef
/
Gènes nef
/
Maladie de Basedow
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Humans
Langue:
En
Journal:
Biochem Biophys Res Commun
Année:
1991
Type de document:
Article