MALDI reveals membrane lipid profile reversion in MDX mice.
Neurobiol Dis
; 36(2): 252-8, 2009 Nov.
Article
de En
| MEDLINE
| ID: mdl-19632329
ABSTRACT
Duchenne muscular dystrophy (DMD), the most common and severe X-linked myopathy, is characterized by the lack of dystrophin, a sub-sarcolemmal protein necessary for normal muscle functions. In a previous study of the lipid content of skeletal muscles of dystrophic (mdx) mice, the animal model for DMD, by in situ Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry (MALDI-MS), an inversion of the phosphatidylcholine PC342/PC341 ion peaks intensity ratio was observed between destructured (abnormal fiber morphology) and structured (normal fiber morphology). A possible treatment for this dramatic disease is to introduce an exogenous nitric oxide (NO) donor into the organism, leading to an increase of utrophin and a regression of the dystrophic phenotype. In the present work, after confirmation by tandem mass spectrometry of the structure of these two phospholipids, their intensity ratio inversion was used to evidence a restoration of membrane lipid composition very similar to those of wild-type mice after the treatment of mdx mice with molsidomine, a NO donor. This was associated with the observation by immunohistology of an increase of the regeneration process in the mice.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Souris de lignée mdx
/
Spectrométrie de masse MALDI
/
Lipides membranaires
Limites:
Animals
Langue:
En
Journal:
Neurobiol Dis
Sujet du journal:
NEUROLOGIA
Année:
2009
Type de document:
Article
Pays d'affiliation:
France