Low susceptibility to N-ethyl-N-nitrosourea-induced transplacental carcinogenesis in Long-Evans Cinnamon (LEC) rats.
J Med Invest
; 56(3-4): 93-8, 2009 Aug.
Article
de En
| MEDLINE
| ID: mdl-19763020
ABSTRACT
The Long-Evans Cinnamon (LEC) rat, an animal model of Wilson's disease, is resistant to a variety of chemical carcinogenesis except liver and colon. In the present study, N-ethyl-N-nitrosourea (ENU)-induced transplacental carcinogenesis was examined in male and female LEC, Long-Evans Agouti (LEA), a sibling line of the LEC rat, and F344 rats (n=21). ENU was administered to pregnant rats as a single s.c. injection at a dose of 60 mg/kg body weight on the 17th day after conception. Cerebral/spinal gliomas and trigeminal/spinal nerve schwannomas developed in both LEA and F344 rats at 30 weeks of age, but no nervous system tumors developed in LEC rats, the difference being statistically significant. Lung adenomas also developed in LEA and F344 rats, but not in LEC rats. Semiquantitative RT-PCR demonstrated that metallothionein (MT)1a, MT2 and O(6)-methylguanine-DNA methyltransferase (MGMT) mRNA levels in the liver of LEC rats were higher than those in F344 and LEA rats. In addition, Western blot analysis showed that MT (MT1 plus MT2) in the liver of LEC rats was also higher than that in other strains. Present results suggest that high levels of MT and/or MGMT contribute to the resistance to nitrosamine-induced carcinogenesis in LEC rats.
Recherche sur Google
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Cancérogènes
/
1-Éthyl-1-nitroso-urée
/
Échange foetomaternel
Limites:
Animals
/
Pregnancy
Langue:
En
Journal:
J Med Invest
Sujet du journal:
MEDICINA
Année:
2009
Type de document:
Article
Pays d'affiliation:
Japon
Pays de publication:
JAPAN
/
JAPON
/
JAPÃO
/
JP