Differential toxicity of antifungal protein AFP against mutants of Fusarium oxysporum.
Int Microbiol
; 12(2): 115-21, 2009 Jun.
Article
de En
| MEDLINE
| ID: mdl-19784931
ABSTRACT
Antifungal protein (AFP) from Aspergillus giganteus was assayed for toxicity against the Fusarium oxysporum wild-type strain and mutants in genes involved in cell signaling (DeltapacC, pacCc Deltafmk1) or cell-wall biogenesis (DeltachsV, Deltachs7, Deltagas1). The mutants were classified into two groups according to their sensitivity to AFP DeltapacC, Deltagas1 and Deltachs7, which were significantly more resistant to AFP than the wild-type, and pacCC, Deltafmk1 and DeltachsV, which were more sensitive. Western blot analysis revealed increased binding of AFP to the three resistant mutants, DeltapacC, Deltagas1 and Deltachs7, but also to DeltachsV, indicating that differential binding may not be a key determinant for sensitivity. Addition of Ca2+ or K+ dramatically reduced antifungal activity and binding of AFP, suggesting that these cations compete for the same targets as AFP at the surface of the fungal cell.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines fongiques
/
Fusarium
/
Antifongiques
Langue:
En
Journal:
Int Microbiol
Sujet du journal:
MICROBIOLOGIA
Année:
2009
Type de document:
Article
Pays d'affiliation:
Espagne