Secretion of intelectin-1 from malignant pleural mesothelioma into pleural effusion.
Br J Cancer
; 103(4): 517-23, 2010 Aug 10.
Article
de En
| MEDLINE
| ID: mdl-20628387
ABSTRACT
BACKGROUND:
Malignant pleural mesothelioma (MPM) is a rare but fatal tumour. Although most MPM patients show pleural effusion at even the early stage, it is hard to diagnose as MPM at the early stage because a sensitive and reliable diagnostic marker for MPM has not been found in plasma or pleural effusion.METHODS:
In this study, we investigated whether intelectin-1 was specifically contained in MPM cells and the pleural effusion of MPM patient by immunohistochemistry, western blotting, and enzyme-linked immunosorbent assay.RESULTS:
Malignant pleural mesothelioma cell lines, but not lung adenocarcinoma cell lines, secreted intelectin-1. In immunohistochemistry, epithelioid-type MPMs, but neither pleura-invading lung adenocarcinomas nor reactive mesothelial cells near the lung adenocarcinomas, were stained with anti-intelectin antibodies. Pleural effusion of MPM patients contained a higher concentration of intelectin-1 than that of lung cancer patients.CONCLUSION:
These results suggest that detection of intelectin-1 may be useful for a differential diagnosis of epithelioid-type MPM in immunohistochemistry and that a high concentration of intelectin-1 in pleural effusion can be used as a new marker for clinical diagnosis of MPM.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs de la plèvre
/
Marqueurs biologiques tumoraux
/
Cytokines
/
Épanchement pleural malin
/
Lectines
/
Mésothéliome
Limites:
Aged
/
Humans
/
Male
/
Middle aged
Langue:
En
Journal:
Br J Cancer
Année:
2010
Type de document:
Article
Pays d'affiliation:
Japon