Deubiquitylase, deSUMOylase, and deISGylase activity microarrays for assay of substrate preference and functional modifiers.
Mol Cell Proteomics
; 10(1): M110.002402, 2011 Jan.
Article
de En
| MEDLINE
| ID: mdl-20956615
ABSTRACT
Microarray-based proteomics expanded the information potential of DNA arrays to the level of protein translation and interaction, but so far, not much beyond. Although enzymatic activity from immobilized proteins has been reliably studied using surface plasmon resonance, a microarray of catalytically competent enzymes would facilitate high throughput, parallel study of their function. The ability to localize activity from soluble substrates has frustrated development of such an array. Here, we report the novel use of previously developed, highly specific suicide substrates for three families of enzymes deubiquitylases, deSUMOylases, and deISGylases. We show specificity of each family to its cognate substrate, and demonstrate utility of the array in a secondary screen of small molecule inhibitors.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Endopeptidases
/
Ubiquitines
/
Petites protéines modificatrices apparentées à l'ubiquitine
/
Analyse par réseau de protéines
/
Esters
Limites:
Humans
Langue:
En
Journal:
Mol Cell Proteomics
Sujet du journal:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Année:
2011
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique