Seeing the portal in herpes simplex virus type 1 B capsids.
J Virol
; 85(4): 1871-4, 2011 Feb.
Article
de En
| MEDLINE
| ID: mdl-21106752
ABSTRACT
Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron cryomicroscopy to enhance the image contrast of ice-embedded herpes simplex virus type 1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid and, for the first time, show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Capside
/
Herpèsvirus humain de type 1
Limites:
Animals
/
Humans
Langue:
En
Journal:
J Virol
Année:
2011
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique