Determination of firocoxib in equine plasma using high performance liquid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci
; 879(2): 205-8, 2011 Jan 15.
Article
de En
| MEDLINE
| ID: mdl-21185237
ABSTRACT
A new method of analysis has been developed and validated for the determination of firocoxib, a new nonsteroidal anti-inflammatory drug (NSAID) approved for use in horses and dogs to control pain and inflammation associated with osteoarthritis. Following a liquid extraction using ethyl acetatehexane (4060), samples were separated by isocratic reversed-phase HPLC on a Sunfire C(18) column and quantified using UV detection at 290 nm. The mobile phase was a mixture of water with 0.025% trifluoroacetic acid and acetonitrile, with a flow-rate of 1.1 ml/min. The procedure produced a linear curve over the concentration range 5-1500 ng/ml with a lower limit of quantification of 5 ng/ml. Intra- and inter-assay variability was less than 7%. The average recovery was 98%. The method is suitable for the analysis of clinical samples from pharmacokinetic studies and can also be used for small volume sample sizes.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Sulfones
/
4-Butyrolactone
/
Anti-inflammatoires non stéroïdiens
/
Chromatographie en phase liquide à haute performance
/
Equus caballus
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Sujet du journal:
ENGENHARIA BIOMEDICA
Année:
2011
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique