Functional polymorphisms in CD166/ALCAM gene associated with increased risk for breast cancer in a Chinese population.
Breast Cancer Res Treat
; 128(2): 527-34, 2011 Jul.
Article
de En
| MEDLINE
| ID: mdl-21293922
ABSTRACT
Activated Leukocyte Cell Adhesion Molecules (ALCAM, also called CD166, MEMD) are cell surface immunoglobulins that are considered to be prognostic markers for breast cancer. CD166/ALCAM has gained increasing attention because of its significant association with tumor progression and the metastatic spread of breast cancer. Two polymorphisms have been identified in the CD166/ALCAM gene 5'UTR C/T (rs6437585) and 3'UTR A/G (rs11559013). We analyzed the genotypes of 1033 individuals with breast cancer, and 1116 controls; odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using logistic regression. The effects and functions of polymorphisms were examined using luciferase gene expression assays and real-time PCR analyses. Our data demonstrated that individuals with the rs6437585 CT + TT genotype had an OR of 1.38 (95% CI, 1.11-1.72) for developing breast cancer, compared to those with the CC genotype. The T allele increased the risk of breast cancer in a dose-dependent manner (P (trend) < 0.001). However, there were no significant differences found between cases and controls at the rs11559013 A/G site. Additional experiments that we performed, which focused on reporter gene expression driven by CD166/ALCAM promoters, demonstrated that the presence of an rs6437585 T allele led to greater transcriptional activity than the rs6437585 C allele. This was consistent with the increased cancer risk that we observed in our case-control analysis.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Polymorphisme génétique
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Tumeurs du sein
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Marqueurs biologiques tumoraux
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Antigènes CD
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Molécules d'adhérence cellulaire neuronale
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Carcinome canalaire du sein
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Asiatiques
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Protéines foetales
Type d'étude:
Etiology_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Limites:
Adult
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Aged
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Aged80
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Female
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Humans
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Middle aged
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
Breast Cancer Res Treat
Année:
2011
Type de document:
Article