Intra-laboratory validation of the Ridascreen® SET Total kit for detecting staphylococcal enterotoxins SEA to SEE in cheese.
Lett Appl Microbiol
; 52(5): 468-74, 2011 May.
Article
de En
| MEDLINE
| ID: mdl-21299578
ABSTRACT
AIM:
To determine the performance of the Ridascreen® SET Total kit, after sample extraction and concentration by dialysis, with regard to its use in official controls for staphylococcal enterotoxins under European Regulation (EC) No. 2073/2005 modified. This study was conducted on naturally contaminated cheese samples and compared with the results of the previously validated Vidas® SET2 kit. METHODS ANDRESULTS:
The effectiveness of the Ridascreen® SET Total kit on naturally contaminated cheeses was compared to that of the Vidas® SET2 kit by applying the EN ISO 16140 standard. Sensitivity and specificity were also compared using spiked buffer solutions and cheese samples with SEA to SEE toxins.CONCLUSIONS:
This study showed that the Ridascreen® SET Total kit is as effective as the Vidas® SET2 kit. SIGNIFICANCE AND IMPACT OF THE STUDY The Ridascreen® SET Total kit was found to specifically detect SEA to SEE in cheeses. The Ridascreen® SET Total can therefore be used to check the staphylococcal enterotoxin content and ensure consumer protection.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Fromage
/
Entérotoxines
/
Microbiologie alimentaire
Type d'étude:
Diagnostic_studies
Langue:
En
Journal:
Lett Appl Microbiol
Sujet du journal:
MICROBIOLOGIA
Année:
2011
Type de document:
Article
Pays d'affiliation:
France