Molecular analysis and characterization of a urease gene operon from Campylobacter sputorum biovar paraureolyticus.
Folia Microbiol (Praha)
; 56(2): 159-65, 2011 Mar.
Article
de En
| MEDLINE
| ID: mdl-21431911
ABSTRACT
When recombinant plasmid DNA from a genomic DNA library and inverse PCR products of Campylobacter sputorum biovar paraureolyticus LMG17591 strain were analyzed, an approximate 6.5-kb pair region, encoding a urease gene operon, was identified. Within the operon, seven closely spaced and putative open reading frames for ureG, ureH(D), ureA, ureB, ureC, ureE, and ureF were detected in order. A possible overlap was detected between ureG and ureH(D), ureH(D) and ureA, and ureE and ureF. In addition, two putative promoter structures, probable ribosome-binding sites and a putative ρ-independent transcriptional terminator structure were identified. The urease gene operon transcription in the cells was confirmed by the reverse transcription-PCR analysis. A neighbor-joining tree constructed based on the nucleotide sequence information of urease genes showed that C. sputorum biovar paraureolyticus formed a cluster with Arcobacter butzleri, urease-positive thermophilic Campylobacter and some Helicobacter spp., separating those from the other urease-producing bacteria, suggesting a commonly shared ancestry among these organisms.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Opéron
/
Urease
/
Campylobacter sputorum
Limites:
Animals
Langue:
En
Journal:
Folia Microbiol (Praha)
Année:
2011
Type de document:
Article
Pays d'affiliation:
Japon