Two-step binding of transcription factors causes sequential chromatin structural changes at the activated IL-2 promoter.
J Immunol
; 187(6): 3292-9, 2011 Sep 15.
Article
de En
| MEDLINE
| ID: mdl-21832163
ABSTRACT
Most gene promoters have multiple binding sequences for many transcription factors, but the contribution of each of these factors to chromatin remodeling is still unclear. Although we previously found a dynamic change in the arrangement of nucleosome arrays at the Il2 promoter during T cell activation, its timing preceded that of a decrease in nucleosome occupancy at the promoter. In this article, we show that the initial nucleosome rearrangement was temporally correlated with the binding of NFAT1 and AP-1 (Fos/Jun), whereas the second step occurred in parallel with the recruitment of other transcription factors and RNA polymerase II. Pharmacologic inhibitors for activation of NFAT1 or induction of Fos blocked the initial phase in the sequential changes. This step was not affected, however, by inhibition of c-Jun phosphorylation, which instead blocked the binding of the late transcription factors, the recruitment of CREB-binding protein, and the acetylation of histone H3 at lysine 27. Thus, the sequential recruitment of transcription factors appears to facilitate two separate steps in chromatin remodeling at the Il2 locus.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Facteurs de transcription
/
Activation des lymphocytes
/
Transduction du signal
/
Interleukine-2
/
Régions promotrices (génétique)
/
Assemblage et désassemblage de la chromatine
Type d'étude:
Etiology_studies
/
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
J Immunol
Année:
2011
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique