Axenic culture and identification of amastigotes from Sichuan human strain of Chinese Leishmania isolates.
Vet Parasitol
; 183(3-4): 353-5, 2012 Feb 10.
Article
de En
| MEDLINE
| ID: mdl-21880429
ABSTRACT
This work describes a simple method to yield large amounts of the isolate MHOM/CN/90/SC10H2 amastigotes-like forms in axenic cultures using promastigotes as the starting population. The isolate MHOM/CN/90/SC10H2, used in this study, belongs to an undescribed species of Leishmania endemic to hill foci in China. The method describes induced extracellular amastigote transformation of this isolate. The rounded parasite obtained in axenic culture was morphologically similar, even at the ultrastructural level, to intracellular amastigotes. Moreover, the axenic amastigotes remained viable as verified by the stage-specific genes (gp46 and p4 genes) with RT-PCR. A 70-80 kDa protein was recognized by polyclonal antibody HRP-IgG only in axenic-derived amastigotes and not in promastigotes.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Parasitologie
/
Culture axénique
/
Leishmania
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Limites:
Humans
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
Vet Parasitol
Année:
2012
Type de document:
Article
Pays d'affiliation:
Chine