Construction and characterization of a normalized cDNA library from the river snail Bellamya aeruginosa after exposure to copper.
Ecotoxicology
; 21(1): 260-7, 2012 Jan.
Article
de En
| MEDLINE
| ID: mdl-21915736
ABSTRACT
The construction of a normalized cDNA library is a popular tool for identifying novel biomarkers for monitoring environmental pollution. In the present study, a normalized cDNA library was constructed from the river snail Bellamya aeruginosa after exposure to Cu(2+) by using the SMART technique. The titer of the cDNA library was 1.78 × 10(6) pfu/ml, with a recombinant efficiency of 95.8%. In addition, from 6,000 randomly selected and sequenced clones, 5,473 high-quality ESTs were identified. After processing the sequences, 3,961 unigenes representing 897 contigs and 3,064 singlets were obtained with 27.6% redundancy. Analysis of expressed sequenced tags using COG and GO annotation and KEGG pathway data showed that a large group of genes related to growth and development, signal transduction, and defense mechanisms were present in the cDNA library. Based on our findings, this normalized cDNA library will provide a valuable resource for further research on functional genes and ecotoxicology in B. aeruginosa.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Escargots
/
Polluants chimiques de l'eau
/
Banque de gènes
/
Surveillance de l'environnement
/
Cuivre
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Ecotoxicology
Sujet du journal:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Année:
2012
Type de document:
Article
Pays d'affiliation:
Chine