Regionalized Twist1 activity in the forelimb bud drives the morphogenesis of the proximal and preaxial skeleton.
Dev Biol
; 362(2): 132-40, 2012 Feb 15.
Article
de En
| MEDLINE
| ID: mdl-22178153
ABSTRACT
Development of the mouse forelimb bud depends on normal Twist1 activity. Global loss of Twist1 function before limb bud formation stops limb development and loss of Twist1 throughout the mesenchyme after limb bud initiation leads to polydactyly, the ulnarization or loss of the radius and malformations and reductions of the shoulder girdle. Here we show that conditional deletion of Twist1 by Mesp1-Cre in the mesoderm that migrates into the anterior-proximal part of the forelimb bud results in the development of supernumerary digits and carpals, the acquisition of ulna-like characteristics by the radius and malformations of the humerus and scapula. The mirror-like duplications and posteriorization of pre-axial tissues are preceded by disruptions to anterior-posterior Shh, Bmp and Fgf signaling gradients and dysregulation of transcription factors that regulate anterior-posterior limb patterning.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines nucléaires
/
Transduction du signal
/
Plan d'organisation du corps
/
Protéine-1 apparentée à Twist
/
Membre thoracique
/
Morphogenèse
Limites:
Animals
Langue:
En
Journal:
Dev Biol
Année:
2012
Type de document:
Article
Pays d'affiliation:
Australie