Differential expression of connective tissue growth factor and extracellular matrix proteins in lichen sclerosus.
J Eur Acad Dermatol Venereol
; 26(2): 207-12, 2012 Feb.
Article
de En
| MEDLINE
| ID: mdl-22280508
ABSTRACT
BACKGROUND:
The histopathology of lichen sclerosus (LS) suggests abnormalities in extracellular matrix (ECM) composition.OBJECTIVES:
We aimed to investigate the expression pattern of ECM proteins and related growths factors and Smad signal transducers in LS as compared with healthy skin.METHODS:
To assess the expression of decorin, biglycan, versican, perlecan, fibronectin, dermatopontin, extracellular matrix protein 1 (ECM-1), matrix metalloproteinase 1, tissue inhibitor of metalloproteinase 1, connective tissue growth factor (CTGF), transforming growth factor ß1, and Smad-3 protein, real-time RT-PCR and immunohistochemistry were performed on skin specimens obtained from the genital region of healthy subjects (n = 10) as well as LS patients (n = 26).RESULTS:
Median mRNA as well as mean protein expression of biglycan, versican, fibronectin, and ECM-1 was significantly higher in LS when compared with healthy controls. Both mRNA and protein CTGF expression observed in LS was significantly higher than in controls. CTGF mRNA expression significantly correlated with mRNA expression of biglycan, versican and fibronectin.CONCLUSIONS:
Expression of ECM proteins (e.g. proteoglycans, ECM-1) and CTGF is altered in LS. TGF-ß/Smad-3 independent up-regulation of CTGF may induce accumulation of ECM proteins and maintain fibrosis in chronic LS.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines de la matrice extracellulaire
/
Lichen scléroatrophique
/
Facteur de croissance du tissu conjonctif
Limites:
Adult
/
Aged
/
Female
/
Humans
/
Middle aged
Langue:
En
Journal:
J Eur Acad Dermatol Venereol
Sujet du journal:
DERMATOLOGIA
/
DOENCAS SEXUALMENTE TRANSMISSIVEIS
Année:
2012
Type de document:
Article
Pays d'affiliation:
Allemagne