Interaction of plectin and intermediate filaments.
J Dermatol Sci
; 66(1): 44-50, 2012 Apr.
Article
de En
| MEDLINE
| ID: mdl-22333906
ABSTRACT
BACKGROUND:
Plectin, a member of the plakin family proteins, is a high molecular weight protein that is ubiquitously expressed. It acts as a cytolinker for the three major components of the cyotoskeleton, namely actin microfilaments, microtubules and intermediate filaments.OBJECTIVE:
The aim of our experiments was to identify new binding sites for intermediate filaments on plectin and to specify these sites.METHODS:
We introduced truncated forms of plectin into several cell lines and observe interaction between plectin and intermediate filaments.RESULTS:
We found that a linker region in the COOH-terminal end of plectin was required for the association of the protein with intermediate filaments. In addition, we also demonstrated that a serine residue at position 4645 of plectin may have a role on binding of plectin to intermediate filaments.CONCLUSION:
A linker region in the COOH-terminal end and serine residue at position 4645 may be important for the binding of plectin to intermediate filaments.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Filaments intermédiaires
/
Plectine
Limites:
Animals
Langue:
En
Journal:
J Dermatol Sci
Sujet du journal:
DERMATOLOGIA
Année:
2012
Type de document:
Article
Pays d'affiliation:
Japon