A pharmacodynamic model of portal hypertension in isolated perfused rat liver.
World J Gastroenterol
; 18(5): 472-8, 2012 Feb 07.
Article
de En
| MEDLINE
| ID: mdl-22346254
ABSTRACT
AIM:
To develop a pharmacodynamic model of portal hypertension from chronic hepatitis.METHODS:
Pathological changes and collagen depositions were analyzed using morphometry to confirm CCl4-induced chronic hepatitis. At d0, d28, d56 and d84 of the process, the portal perfused velocities (µL/min) in isolated rat livers were exactly controlled with a quantified pump. The pressure (mmHg) was monitored with a Physiological System. The geometric concentrations of phenylephrine or acetylcholine were added to a fixed volume (300 mL) of the circulating perfusate. The equation, the median effective concentration and its 95% confidence intervals of phenylephrine or acetylcholine were regressed with Prism-4 software in non-linear fit and various slopes. In the isolated perfused rat livers with chronic hepatitis, both median effective concentrations were defined as the pharmacodynamic model of portal hypertension.RESULTS:
At d0, d28, d56 and d84, the equations of portal pressure potency from the concentrations of phenylephrine used to constrict the portal vein in isolated perfused rat livers were Y = 0.1732 + 0.3970/[1 + 10((-4.3061-0.4407 X))], Y = -0.004934 + 0.12113/[1 + 10((-3.1247-0.3262 X))], Y = 0.0104 + 0.2643/[1 + 10((-8.8462-0.9579 X))], and Y = 0.01603 + 0.12107/[1 + 10((-5.1134-0.563 X))]; the median effective concentrations were 1.69 × 10⻹° mol/L, 2.64 × 10⻹° mol/L, 5.82 × 10⻹° mol/L, and 8.24 × 10⻹° mol/L, respectively. The equations from the concentrations of acetylcholine used to relax the portal vein were Y = -0.4548 + 0.3274/[1 + 10((6.1538 + 0.5554 X))], Y = -0.05391 + 0.06424/[1 + 10((3.8541 + 0.3469 X))], Y = -0.2733 + 0.22978/[1 + 10((3.0472 + 0.3008 X))], and Y = -0.0559 + 0.053178/[1 + 10((5.6336 + 0.5883 X))]; the median effective concentrations were 8.40 × 10⻹° mol/L, 7.73 × 10⻹² mol/L, 5.98 × 10⻹¹ mol/L, and 2.66 × 10⻹° mol/L, respectively.CONCLUSION:
A pharmacodynamic model of portal hypertension in isolated perfused rat livers with chronic hepatitis was defined as the median effective concentrations of phenylephrine and acetylcholine.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Veine porte
/
Modèles animaux de maladie humaine
/
Hypertension portale
/
Foie
Type d'étude:
Clinical_trials
/
Etiology_studies
Limites:
Animals
/
Humans
/
Male
Langue:
En
Journal:
World J Gastroenterol
Sujet du journal:
GASTROENTEROLOGIA
Année:
2012
Type de document:
Article
Pays d'affiliation:
Chine