Involvement of the prostate and testis expression (PATE)-like proteins in sperm-oocyte interaction.
Hum Reprod
; 27(5): 1238-48, 2012 May.
Article
de En
| MEDLINE
| ID: mdl-22402205
ABSTRACT
BACKGROUND:
The prostate and testis expression (PATE)-like family of proteins are expressed mainly in the male genital tract. They are localized in the sperm head and are homologous to SP-10, the acrosomal vesicle protein also named ACRV1. Our aim was to characterize the expression and functional role of three PATE-like proteins in the testis and ejaculated sperm.METHODS:
The expression and localization of PATE-like proteins in human testis biopsies (n= 95) and sperm cells were assessed by RT-PCR, immunohistochemistry and immunofluorescence staining (at least 600 sperm cells per specimen). The function of the PATE protein was tested by the hemizona assay and hamster egg penetration test (HEPT).RESULTS:
PATE and PATE-M genes and proteins were present almost exclusively in germ cells in the testis immunoflourescence showed that the percentage of germ cells positive for PATE, PATE-M and PATE-B was 85, 50 and 2%, respectively. PATE and PATE-M proteins were localized in the equatorial segment of the sperm head, while PATE-B protein was localized in the post-acrosomal region. A polyclonal antibody (Ab, at 150 and 1200 dilutions) against the PATE protein did not inhibit sperm-zona binding in the hemizona assay (hemizona index of 89.6 ± 10 and 87 ± 36%, respectively). However, there was inhibition of sperm-oolemma fusion and penetration in the HEPT (penetration index without Ab 7 ± 3.9; Ab dilution of 1100, 4 ± 3.5; Ab dilution of 120, 0.6 ± 1.2, P < 0.001).CONCLUSIONS:
Our data suggest that PATE protein is involved in sperm-oolemma fusion and penetration but not sperm-zona binding.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Interaction sperme-ovule
/
Protéines membranaires
Limites:
Animals
/
Female
/
Humans
/
Male
Langue:
En
Journal:
Hum Reprod
Sujet du journal:
MEDICINA REPRODUTIVA
Année:
2012
Type de document:
Article
Pays d'affiliation:
Israël