Your browser doesn't support javascript.
loading
Genetic polymorphisms in bovine transferrin receptor 2 (TFR2) and solute carrier family 40 (iron-regulated transporter), member 1 (SLC40A1) genes and their association with beef iron content.
Duan, Q; Tait, R G; Mayes, M S; Garrick, D J; Liu, Q; Van Eenennaam, A L; Mateescu, R G; Van Overbeke, D L; Garmyn, A J; Beitz, D C; Reecy, J M.
Affiliation
  • Duan Q; Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
Anim Genet ; 43(2): 115-22, 2012 Apr.
Article de En | MEDLINE | ID: mdl-22404347
ABSTRACT
Beef is considered to be an excellent source of dietary iron. However, little is known about the genetic control of beef iron content. We hypothesized that genetic polymorphisms in transferrin receptor 2 (TFR2) and solute carrier family 40 (iron-regulated transporter), member 1 (SLC40A1) could influence skeletal muscle iron content. The objective of this study was to use Angus cattle to identify single-nucleotide polymorphisms (SNPs) in the exons and flanking regions of the bovine TFR2 and SLC40A1 genes and to evaluate the extent to which genetic variation in them was associated with bovine longissimus dorsi muscle iron content. Ten novel SNPs were identified in TFR2, of which one SNP tended to be associated (P < 0.013) with skeletal muscle iron content. Nine novel SNPs in SLC40A1, NC007300 rs133108154, rs137140497, rs135205621, rs136600836, rs134388440, rs136347850, rs134186279, rs134621419 and rs137555693, were identified, of which SNPs rs134388440, rs136347850 and rs137555693 were significantly associated (P < 0.007) with skeletal muscle iron content. High linkage disequilibrium was observed among SLC40A1 SNPs rs134388440, rs136347850 and rs137555693 (R(2) > 0.99), from which two haplotypes, TGC and CAT, were defined. Beef from individuals that were homozygous for the TGC haplotype had significantly (P < 0.001) higher iron content than did beef from CAT homozygous or heterozygous individuals. The estimated size of effect of the identified haplotypes was 0.3% of the phenotypic variance. In conclusion, our study provides evidence for genetic control of beef iron concentration. Moreover, SNPs identified in SLC40A1, rs134388440, rs136347850 and rs137555693 might be useful markers for the selection of Angus cattle for altered iron content.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Récepteurs à la transferrine / Bovins / Polymorphisme de nucléotide simple / Transporteurs de cations / Fer / Viande Type d'étude: Risk_factors_studies Limites: Animals Langue: En Journal: Anim Genet Sujet du journal: GENETICA / MEDICINA VETERINARIA Année: 2012 Type de document: Article Pays d'affiliation: États-Unis d'Amérique

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Récepteurs à la transferrine / Bovins / Polymorphisme de nucléotide simple / Transporteurs de cations / Fer / Viande Type d'étude: Risk_factors_studies Limites: Animals Langue: En Journal: Anim Genet Sujet du journal: GENETICA / MEDICINA VETERINARIA Année: 2012 Type de document: Article Pays d'affiliation: États-Unis d'Amérique