The UDP glucose: flavonoid-3-O-glucosyltransferase (UFGT) gene regulates anthocyanin biosynthesis in litchi (Litchi chinesis Sonn.) during fruit coloration.
Mol Biol Rep
; 39(6): 6409-15, 2012 Jun.
Article
de En
| MEDLINE
| ID: mdl-22447536
ABSTRACT
We analyzed a litchi cultivar that included three phenotypes for pericarp color, ranging from green, indicating the absence of anthocyanins, to yellow, and red. Anthocyanins, chlorophylls, carotenoids, and flavonoids were measured in the three stages. Fruit coloration of red-skinned litchi was mainly due to higher flavonols, and anthocyanin pigments, lower chlorophyll (higher chlorophyll degradation). Expression of four genes of the anthocyanin pathway coding for phenylalanine ammonialyase, chalcone synthase, flavanone-3-hydroxylase, and the UDP-glucose flavonoid-3-O-glucosyltransferase (UFGT), was analyzed by RT-PCR at three developmental stages from before the onset of ripening to full maturity. Gene expression patterns were compared to anthocyanin metabolites. The contents of anthocyanins and flavonols in the pericarps were consistent with the higher mRNA levels of UFGT, while, transcription of the other gene was not expected to follow the anthocyanin content. We suggest that UFGT might play an important role in anthocyanin biosynthesis in the pericarp of litchi. Thus, UFGT expression strongly influences fruit coloration in litchi.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines végétales
/
Pigmentation
/
Litchi
/
Fruit
/
Glucosyltransferases
/
Anthocyanes
Langue:
En
Journal:
Mol Biol Rep
Année:
2012
Type de document:
Article
Pays d'affiliation:
Chine