Host invasion by Batrachochytrium dendrobatidis: fungal and epidermal ultrastructure in model anurans.
Dis Aquat Organ
; 100(3): 201-10, 2012 Sep 12.
Article
de En
| MEDLINE
| ID: mdl-22968788
ABSTRACT
The chytridiomycete fungus Batrachochytrium dendrobatidis (Bd) colonizes mouthparts of amphibian larvae and superficial epidermis of post-metamorphic amphibians, causing the disease chytridiomycosis. Fungal growth within host cells has been documented by light and transmission electron microscopy; however, entry of the fungus into host cells has not. Our objective was to document how Bd enters host cells in the wood frog Lithobates sylvaticus, a species at high mortality risk for chytridiomycosis, and the bullfrog L. catesbeianus, a species at low mortality risk for chytridiomycosis. We inoculated frogs and documented infection with transmission electron microscopy. Zoospores encysted on the skin surface and produced morphologically similar germination tubes in both host species that penetrated host cell membranes and enabled transfer of zoospore contents into host cells. Documenting fungal and epidermal ultrastructure during host invasion furthers our understanding of Bd development and the pathogenesis of chytridiomycosis.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Anura
/
Chytridiomycota
/
Épiderme
Limites:
Animals
Langue:
En
Journal:
Dis Aquat Organ
Sujet du journal:
BIOLOGIA
/
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Année:
2012
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique