Proteomic analysis of the mice hippocampus after preconditioning induced by N-methyl-D-aspartate (NMDA).
J Mol Neurosci
; 50(1): 154-64, 2013 May.
Article
de En
| MEDLINE
| ID: mdl-23001814
ABSTRACT
Preconditioning induced by N-methyl-D-aspartate (NMDA) has been used as a therapeutic tool against later neuronal insults. NMDA preconditioning affords neuroprotection against convulsions and cellular damage induced by the NMDA receptor agonist, quinolinic acid (QA) with time-window dependence. This study aimed to evaluate the molecular alterations promoted by NMDA and to compare these alterations in different periods of time that are related to the presence or lack of neuroprotection. Putative mechanisms related to NMDA preconditioning were evaluated via a proteomic analysis by using a time-window study. After a subconvulsant and protective dose of NMDA administration mice, hippocampi were removed (1, 24 or 72 h) and total protein analyzed by 2DE gels and identified by MALDI-TOF. Differential protein expression among the time induction of NMDA preconditioning was observed. In the hippocampus of protected mice (24 h), four proteins HSP70(B), aspartyl-tRNA synthetase, phosphatidylethanolamine binding protein and creatine kinase were found to be up-regulated. Two other proteins, HSP70(A) and V-type proton ATPase were found down-regulated. Proteomic analysis showed that the neuroprotection induced by NMDA preconditioning altered signaling pathways, cell energy maintenance and protein synthesis and processing. These events may occur in a sense to attenuate the excitotoxicity process during the activation of neuroprotection promoted by NMDA preconditioning.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
N-Méthyl-aspartate
/
Neuroprotecteurs
/
Protéomique
/
Hippocampe
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
J Mol Neurosci
Sujet du journal:
BIOLOGIA MOLECULAR
/
NEUROLOGIA
Année:
2013
Type de document:
Article
Pays d'affiliation:
Brésil