Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes.
Adv Mater
; 25(11): 1598-602, 2013 Mar 20.
Article
de En
| MEDLINE
| ID: mdl-23315653
ABSTRACT
A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Langue:
En
Journal:
Adv Mater
Sujet du journal:
BIOFISICA
/
QUIMICA
Année:
2013
Type de document:
Article
Pays d'affiliation:
Allemagne