Identification of transcriptional regulators in the mouse immune system.
Nat Immunol
; 14(6): 633-43, 2013 Jun.
Article
de En
| MEDLINE
| ID: mdl-23624555
ABSTRACT
The differentiation of hematopoietic stem cells into cells of the immune system has been studied extensively in mammals, but the transcriptional circuitry that controls it is still only partially understood. Here, the Immunological Genome Project gene-expression profiles across mouse immune lineages allowed us to systematically analyze these circuits. To analyze this data set we developed Ontogenet, an algorithm for reconstructing lineage-specific regulation from gene-expression profiles across lineages. Using Ontogenet, we found differentiation stage-specific regulators of mouse hematopoiesis and identified many known hematopoietic regulators and 175 previously unknown candidate regulators, as well as their target genes and the cell types in which they act. Among the previously unknown regulators, we emphasize the role of ETV5 in the differentiation of γδ T cells. As the transcriptional programs of human and mouse cells are highly conserved, it is likely that many lessons learned from the mouse model apply to humans.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Transcription génétique
/
Algorithmes
/
Régulation de l'expression des gènes
/
Système immunitaire
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Animals
/
Humans
Langue:
En
Journal:
Nat Immunol
Sujet du journal:
ALERGIA E IMUNOLOGIA
Année:
2013
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique