Genetic analysis of health-related secondary metabolites in a Brassica rapa recombinant inbred line population.
Int J Mol Sci
; 14(8): 15561-77, 2013 Jul 25.
Article
de En
| MEDLINE
| ID: mdl-23892600
ABSTRACT
The genetic basis of the wide variation for nutritional traits in Brassica rapa is largely unknown. A new Recombinant Inbred Line (RIL) population was profiled using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) analysis to detect quantitative trait loci (QTLs) controlling seed tocopherol and seedling metabolite concentrations. RIL population parent L58 had a higher level of glucosinolates and phenylpropanoids, whereas levels of sucrose, glucose and glutamate were higher in the other RIL population parent, R-o-18. QTL related to seed tocopherol (α-, ß-, γ-, δ-, α-/γ- and total tocopherol) concentrations were detected on chromosomes A3, A6, A9 and A10, explaining 11%-35% of the respective variation. The locus on A3 co-locates with the BrVTE1gene, encoding tocopherol cyclase. NMR spectroscopy identified the presence of organic/amino acid, sugar/glucosinolate and aromatic compounds in seedlings. QTL positions were obtained for most of the identified compounds. Compared to previous studies, novel loci were found for glucosinolate concentrations. This work can be used to design markers for marker-assisted selection of nutritional compounds in B. rapa.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Brassica rapa
/
Tocophérols
/
Glucosinolates
/
Valeur nutritive
Type d'étude:
Prognostic_studies
Langue:
En
Journal:
Int J Mol Sci
Année:
2013
Type de document:
Article
Pays d'affiliation:
Pays-Bas