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In situ visualization of telomere elongation patterns in human cells.
Diolaiti, Morgan E; Cimini, Beth A; Kageyama, Robin; Charles, Florie A; Stohr, Bradley A.
Affiliation
  • Diolaiti ME; Department of Pathology, University of California, San Francisco, CA 94143, USA and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.
Nucleic Acids Res ; 41(18): e176, 2013 Oct.
Article de En | MEDLINE | ID: mdl-23963699
The telomerase enzyme plays a critical role in human aging and cancer biology by maintaining telomere length and extending the proliferative lifespan of most stem cells and cancer cells. Despite the importance of this enzyme, our understanding of the mechanisms that regulate its activity and establish telomere length homeostasis in mammalian cells is incomplete, in part because the perfect repetitive nature of telomeric sequence hampers in situ detection of telomere elongation patterns. Here, we describe a novel assay using a mutant telomerase that adds a well-tolerated variant telomeric repeat sequence to telomere ends. By specifically detecting the addition of these variant repeats, we can directly visualize telomere elongation events in human cells. We validate this approach by in situ mapping of telomere elongation patterns within individual nuclei and across a population of cells.
Sujet(s)

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Homéostasie des télomères Limites: Humans Langue: En Journal: Nucleic Acids Res Année: 2013 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: Royaume-Uni

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Homéostasie des télomères Limites: Humans Langue: En Journal: Nucleic Acids Res Année: 2013 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: Royaume-Uni