Wafer-scale nanowell array patterning based electrochemical impedimetric immunosensor.
J Biotechnol
; 168(4): 584-8, 2013 Dec.
Article
de En
| MEDLINE
| ID: mdl-24013070
ABSTRACT
We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In this research, we developed an impedimetric immunosensor based on wafer-scale NWA for electrochemical detection of stress-induced-phosphoprotein-1 (STIP-1). In order to develop NWA sensor through the cost-effective combination of high-throughput nanopattern, the NWA electrode was fabricated on Si wafer by krypton-fluoride (KrF) stepper semiconductor process. Finally, 12,500,000 ea nanowell with a 500 nm diameter was fabricated on 4 mm × 2 mm substrate. Next, by using these electrodes, we measured impedance to quantify antigen binding to the immunoaffinity layer. The limit of detection (LOD) of the NWA was improved about 100-fold compared to milli-sized electrodes (4 mm × 2 mm) without an NWA. These results suggest that wafer-scale NWA immunosensor will be useful for biosensing applications because their interface response is appropriate for detecting molecular binding events.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Sites de fixation
/
Techniques de biocapteur
/
Protéines du choc thermique
Limites:
Humans
Langue:
En
Journal:
J Biotechnol
Sujet du journal:
BIOTECNOLOGIA
Année:
2013
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique