Halomonas olivaria sp. nov., a moderately halophilic bacterium isolated from olive-processing effluents.
Int J Syst Evol Microbiol
; 64(Pt 1): 46-54, 2014 Jan.
Article
de En
| MEDLINE
| ID: mdl-24030688
ABSTRACT
A moderately halophilic, Gram-stain-negative, non-sporulating bacterium designed as strain TYRC17(T) was isolated from olive-processing effluents. The organism was a straight rod, motile by means of peritrichous flagella and able to respire both oxygen and nitrate. Growth occurred with 0-25â% (w/v) NaCl (optimum, 7â%), at pH 5-11 (optimum, pH 7.0) and at 4-50 °C (optimally at 35 °C). It accumulated poly-ß-hydroxyalkanoate granules and produced exopolysaccharides. The predominant fatty acids were C18ââ1ω7c, C16ââ1ω7c and C16ââ0. Ubiquinone 9 (Q-9) was the only respiratory quinone. The DNA G+C content of TYRC17(T) was 53.9 mol%. Phylogenetic analyses of 16S rRNA gene sequences revealed that the strain represents a member of the genus Halomonas and more precisely of the subgroup containing Halomonas sulfidaeris, H. titanicae, H. variabilis, H. zhanjiangensis, H. alkaliantarctica, H. boliviensis and H. neptunia. TYRC17(T) showed high 16S-rRNA sequence identities in particular with the three last species listed (99.4-99.5â%). A multilocus sequence analysis (MLSA) using the 23S rRNA, gyrB, rpoD and secA genes allowed clarifying the phylogenetic position of TYRC17(T). This, combined with the level of DNA-DNA hybridization between TYRC17(T) and its closest relatives ranging from 21.6â% to 48.4â%, indicated that TYRC17(T) did not represent any of these species. On the basis of phenotypic and genotypic characteristics, and also genomic and phylogenetic evidence, it was concluded that strain TYRC17(T) represented a novel species of the genus Halomonas. The name Halomonas olivaria sp. nov. is proposed with TYRC17(T) (â=âDSM 19074(T)â=âCCUG 53850B(T)) as the type strain.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Phylogenèse
/
Halomonas
/
Olea
/
Eaux usées
Type d'étude:
Prognostic_studies
Pays/Région comme sujet:
Africa
Langue:
En
Journal:
Int J Syst Evol Microbiol
Sujet du journal:
MICROBIOLOGIA
Année:
2014
Type de document:
Article