A new enzyme immunoassay for prolactin in serum or plasma.
Clin Chem
; 36(1): 76-80, 1990 Jan.
Article
de En
| MEDLINE
| ID: mdl-2404642
ABSTRACT
This enzyme immunoassay (EIA) of human prolactin (hPRL) involves incubation of sample and anti-hPRL antibodies conjugated to horseradish peroxidase (EC 1.11.1.7) in tubes coated with a second antibody to hPRL. The test can be performed within 60 min. No reaction of the antibodies with human placental lactogen and human somatotropin is detectable. The presence of detergent allows assay of both serum and plasma. Precision was improved by including polyethylene glycol in the reaction mixture. To optimize analytical recovery, we added protease inhibitor. Assay of the EIA standards shows good correlation with results for World Health Organization reference preparations. The measurable range is 1 to 400 micrograms/L. Intra- and interassay CVs are about 5%. Comparisons with two RIAs and two other EIAs show reasonably good correlations. The components of our EIA are stable for 18 months.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Peroxidases
/
Prolactine
/
Horseradish peroxidase
Limites:
Humans
Langue:
En
Journal:
Clin Chem
Sujet du journal:
QUIMICA CLINICA
Année:
1990
Type de document:
Article