MALDI-TOF mass spectrometry as a tool for differentiation of Bradyrhizobium species: application to the identification of Lupinus nodulating strains.
Syst Appl Microbiol
; 36(8): 565-71, 2013 Dec.
Article
de En
| MEDLINE
| ID: mdl-24168963
ABSTRACT
Genus Bradyrhizobium includes slow growing bacteria able to nodulate different legumes as well as species isolated from plant tumours. The slow growth presented by the members of this genus and the phylogenetic closeness of most of its species difficults their identification. In the present work we applied for the first time Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) to the analysis of Bradyrhizobium species after the extension of MALDI Biotyper 2.0 database with the currently valid species of this genus. With this methodology it was possible to identify strains belonging to phylogenetically closely related species of genus Bradyrhizobium allowing the discrimination among species with rrs gene identities higher than 99%. The application of MALDI-TOF MS to strains isolated from nodules of different Lupinus species in diverse geographical locations allowed their correct identification when comparing with the results of rrs gene and ITS analyses. The nodulation of Lupinus gredensis, an endemic species of the west of Spain, by B. canariense supports the European origin of this species.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Techniques bactériologiques
/
Spectrométrie de masse MALDI
/
Bradyrhizobium
/
Lupinus
/
Nodules racinaires de plante
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Pays/Région comme sujet:
Europa
Langue:
En
Journal:
Syst Appl Microbiol
Année:
2013
Type de document:
Article
Pays d'affiliation:
Espagne