Plant regeneration from protoplast culture of sweet potato (Ipomoea batatas Lam.).

ABSTRACT

This is the first report on successful plant regeneration from protoplasts of sweet potato. Two cultivars (Guyana and Duclos XI) of sweet potato plants propagated under in vitro conditions were used as the source of protoplasts. Green compact calli with meristematic areas were induced in the medium supplemented with 2mg1(-1) zeatin, and plant regeneration occurred when these calli were transferred onto the medium with zeatin level reduced to 0.25mg1(-1). Plant regeneration was found to be genotype-dependent, since it was only obtained for cultivar Duclos XI.