Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria.
Biosens Bioelectron
; 54: 378-84, 2014 Apr 15.
Article
de En
| MEDLINE
| ID: mdl-24292143
ABSTRACT
Blood safety is a global health goal. In developed countries, bacterial contamination of platelet concentrates is the highest infectious risk in transfusion despite the current preventive strategies. We aimed to develop a conductometric biosensor for the generic, rapid and sensitive detection of Gram-negative bacteria. Our strategy is based on immunosensors addressable magnetic nanoparticles coupled with anti-LPS antibodies were used for the generic capture of Gram-negative bacteria. Bacterial capture was characterized by impedancemetric and microscopic measurements. The results obtained with conductometric measurements allowed real-time, sensitive detection of Escherichia coli or Serratia marcescens cultures from 1 to 10(3) CFU mL(-1). The ability of the immunosensor to detect Gram negative bacteria was also tested on clinically relevant strains. The conductometric immunosensor allowed the direct detection of 10-10(3) CFU mL(-1) of Pseudomonas aeruginosa and Acinetobacter baumannii strains that were undetectable using standard immunoblot methods. Results showed that the conductometric response was not inhibited in 1% serum.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Dosage immunologique
/
Techniques de biocapteur
/
Conductimétrie
/
Anticorps immobilisés
/
Nanoparticules de magnétite
/
Bactéries à Gram négatif
Type d'étude:
Diagnostic_studies
/
Evaluation_studies
Limites:
Humans
Langue:
En
Journal:
Biosens Bioelectron
Sujet du journal:
BIOTECNOLOGIA
Année:
2014
Type de document:
Article
Pays d'affiliation:
France