Kmasker--a tool for in silico prediction of single-copy FISH probes for the large-genome species Hordeum vulgare.
Cytogenet Genome Res
; 142(1): 66-78, 2014.
Article
de En
| MEDLINE
| ID: mdl-24335088
ABSTRACT
Specific localization of large genomic fragments by fluorescence in situ hybridization (FISH) is challenging in large- genome plant species due to the high content of repetitive sequences. We report the automated work flow (Kmasker) for in silico extraction of unique genomic sequences of large genomic fragments suitable for FISH in barley. This method can be widely used for the integration of genetic and cytogenetic maps in plants and other species with large and complex genomes if the probe sequence (e.g. BACs, sequence contigs) and a low coverage (8-fold) of unassembled sequences of the species of interest are available. Kmasker has been made publicly available as a web tool at http//webblast.ipk-gatersleben.de/kmasker.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Hordeum
/
Simulation numérique
/
Logiciel
/
Séquences répétées d'acides nucléiques
/
Sondes d'ADN
/
Hybridation fluorescente in situ
/
Génome végétal
/
ADN des plantes
/
Modèles génétiques
Type d'étude:
Prognostic_studies
/
Risk_factors_studies
Langue:
En
Journal:
Cytogenet Genome Res
Sujet du journal:
GENETICA
Année:
2014
Type de document:
Article
Pays d'affiliation:
Allemagne