[Synthesis and experimental study of a novel polymer/gene compound drug controlled release system for the treatment of erectile dysfunction].
Zhonghua Nan Ke Xue
; 19(9): 771-5, 2013 Sep.
Article
de Zh
| MEDLINE
| ID: mdl-24386851
ABSTRACT
OBJECTIVE:
To overcome the deficiency in the current therapies for erectile dysfunction (ED), we designed and synthesized a novel high-efficiency polymer/gene compound drug controlled release system and discussed the feasibility of pH and temperature dually sensitive injectable hydrogel in ED gene therapy.METHODS:
We synthesized optimal siRNA gene nanoparticles by characterizing the zeta potential of polylysine (PLL)/siRNA gene compounds, and established a pH and temperature dually sensitive injectable gene compound drug controlled release system via Schiffs reaction between glycol chitosan (GC) and benzaldehyde capped OHC-PEO-PPO-PEO-CHO. Then we demonstrated the sustained release of the system at different temperatures.RESULTS:
When the mass ratio of PLL to siRNA was 201, the zeta potential of the PLL/siRNA gene compound reached the peak (+23.5 mV) and the siRNA was encapsulated by PLL in the maximal degree. GC and OHC-PEO-PPO-PEO-CHO was crosslinked via benzoicimine reaction when environmental pH was changed from 5.5 to 7.4. The reslease of the siRNA encapsulated in this system kept at a low rate at 37 degrees C, significantly enhanced with the increase of the temperature to 60 degrees C, rising to (122.5 +/- 5.3) microg at 1 000 minutes as compared with (23.8 +/- 6.0) microg at 37 degrees C (P < 0.05).CONCLUSION:
The polymer/gene compound drug controlled release system was successfully synthesized, which improved the stability and capacity of gene carriers and achieved siRNA release at different temperatures, promising to be a new approach to the gene therapy of ED.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Systèmes de délivrance de médicaments
/
Préparations à action retardée
/
Dysfonctionnement érectile
Limites:
Humans
/
Male
Langue:
Zh
Journal:
Zhonghua Nan Ke Xue
Sujet du journal:
MEDICINA REPRODUTIVA
Année:
2013
Type de document:
Article
Pays d'affiliation:
Chine