Cephalometric assessment of craniofacial dysmorphologies in relation with Msx2 mutations in mouse.
Orthod Craniofac Res
; 17(2): 92-105, 2014 May.
Article
de En
| MEDLINE
| ID: mdl-24387797
ABSTRACT
OBJECTIVES:
To determine the role of Msx2 in craniofacial morphology and growth, we used a mouse model and performed a quantitative morphological characterization of the Msx2 (-/-) and the Msx2 (+/-) phenotype using a 2D cephalometric analysis applied on micrographs. MATERIALS ANDMETHODS:
Forty-four three-and-a-half-month-old female CD1 mice were divided into the following three groups Msx2 (+/+) (n = 16), Msx2 (+/-) (n = 16), and Msx2 (-/-) (n = 12). Profile radiographs were scanned. Modified cephalometric analysis was performed to compare the three groups.RESULTS:
Compared with the wild-type mice, the Msx2 (-/-) mutant mice presented an overall craniofacial size decrease and modifications of the shape of the different parts of the craniofacial skeleton, namely the neurocranium, the viscerocranium, the mandible, and the teeth. In particular, dysmorphologies were seen in the cochlear apparatus and the teeth (taurodontism, reduced incisor curvature). Finally contrary to previous published results, we were able to record a specific phenotype of the Msx2 (+/-) mice with this methodology. This Msx2 (+/-) mouse phenotype was not intermediate between the Msx2 (-/-) and the wild-type animals.CONCLUSION:
Msx2 plays an important role in craniofacial morphogenesis and growth because almost all craniofacial structures were affected in the Msx2(-/-) mice including both intramembranous and endochondral bones, the cochlear apparatus, and the teeth. In addition, Msx2 haploinsufficiency involves a specific phenotype with subtle craniofacial structures modifications compared with human mutations.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Céphalométrie
/
Protéines à homéodomaine
/
Malformations crâniofaciales
/
Mutation
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Animals
/
Female
/
Humans
Langue:
En
Journal:
Orthod Craniofac Res
Sujet du journal:
ODONTOLOGIA
/
ORTODONTIA
Année:
2014
Type de document:
Article
Pays d'affiliation:
France