Role of insulin-like growth factor II receptor in transdifferentiation of free silica-induced primary rat lung fibroblasts.
Biomed Environ Sci
; 26(12): 979-85, 2013 Dec.
Article
de En
| MEDLINE
| ID: mdl-24393507
ABSTRACT
OBJECTIVE:
To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts.METHODS:
Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.RESULTS:
The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.CONCLUSION:
The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Différenciation cellulaire
/
Récepteur IGF de type 2
/
Silice
/
Poumon
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Biomed Environ Sci
Sujet du journal:
SAUDE AMBIENTAL
Année:
2013
Type de document:
Article
Pays d'affiliation:
Chine